美国原装进口SIGMA矿物油/石蜡油Mineral oil M8410
矿物油/石蜡油(未灭菌) SIGMA Mineral oil
产品编号 M8410
CAS号 8042-47-5
light oil (neat), BioReagent, suitable for mouse embryo cell culture
M8410-5ML
M8410-100ML
M8410-500ML
M8410-1L
Analysis Note
This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.)
Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing
embryo manipulations.
1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA.
For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours.
For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material. |