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Invitrogen™ M-MLV Reverse Transcriptase反转录酶28025-021
M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a
complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
• Thermostability – Optimal activity at 37°C
• Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)
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Shipping Condition: |
Wet Ice |
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Optimal Reaction Temperature: |
37° C |
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Final Product Size(s): |
7 kb or less |
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Ribonuclease H Activity: |
Reduced |
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Concentration: |
200 U⁄µl |
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Reverse Transcriptase: |
M-MLV Reverse Transcriptase |
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Form: |
Frozen |
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Format: |
Tube(s) |
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Template: |
ssRNA,
RNA-DNA Hybrid,
ssDNA |
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Sensitivity: |
Low |
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Product Size: |
200,000 units |
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Enzyme Function: |
RNA dependent DNA Polymerase |
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Number of Reactions: |
1000 Reactions |
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Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures. | |
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